Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Target IC₅₀: 2-10 nM against human MMP-1

Primary TargetHuman MMP-1

IC₅₀ against native human MMP-1 is in the range of 2-10 nM

General description

Recombinant, human TIMP-2 expressed in CHC cells. Migrates as a 24 kDa protein under reducing conditions on SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-2 is a ~21 kDa (nonreduced) or a ~24 kDa (reduced) protein on SDS-PAGE that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-2 preferentially binds and inhibits MMP-2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. Useful for SDS-PAGE/immunoblot standard and inhibition studies.

M. W.: 24000

Recombinant, human TIMP-2 expressed in CHC cells. Migrates as a 24 kDa protein under reducing conditions on SDS-PAGE. Use 20 µl per gel lane.

Legal Information

Sold under license of U.S. Patents 5,595,885 and 5,698,671. Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Clermont, A., et al. 2004. Biochem. J.382, 315.Boone, T.C., et al. 1990. Proc. Natl. Acad. Sci. USA87, 2800.Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology. Vol. 1(2), 99.Kodama, S., et al. 1987. Collagen Rel. Res.7, 341.Docherty, A.J.P., et al. 1985. Nature318, 66.

Packaging

3 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

Lyophilized from PBS, 100 µg BSA.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Reconstitute by adding 200 µl PBS to the tube and mixing gently until in solution.

Warning

Toxicity: Standard Handling (A)